Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS
cell line
U2OS
cell type
Osteosarcoma
genotype
p53 A347D/A347D
antibody
p53 (Santa Cruz, sc-126)

Sequenced DNA Library

library_name
GSM7074074
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde at room temperature for 10 min, quenched by 125mM glycine,and sonicated in ChIP lysis buffer on ice ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KK8502) according to the manufacture's instruction

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
23051219
Reads aligned (%)
94.9
Duplicates removed (%)
1.3
Number of peaks
670 (qval < 1E-05)

hg19

Number of total reads
23051219
Reads aligned (%)
94.2
Duplicates removed (%)
3.0
Number of peaks
839 (qval < 1E-05)

Base call quality data from DBCLS SRA