Antigen

Antigen Class

TFs and others

Antigen

TP53

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS

cell line

U2OS

cell type

Osteosarcoma

genotype

p53 A347D/A347D

antibody

p53 (Santa Cruz, sc-126)

Sequenced DNA Library

library_name

GSM7074074

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde at room temperature for 10 min, quenched by 125mM glycine,and sonicated in ChIP lysis buffer on ice ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KK8502) according to the manufacture's instruction

Sequencing Platform

instrument_model

NextSeq 550

hg38

Number of total reads

23051219

Reads aligned (%)

94.9

Duplicates removed (%)

1.3

Number of peaks

670 (qval < 1E-05)

hg19

Number of total reads

23051219

Reads aligned (%)

94.2

Duplicates removed (%)

3.0

Number of peaks

839 (qval < 1E-05)